DETERMINATION OF MICROCYSTINS FROM FRESHWATER PONDS IN MYMENSINGH, BANGLADESH
By: Sumaiya Ahmed, Sarah Subhana Islam , Md. Sagir Ahmed
Key Words: Cyanobacteria bloom, microcystins, eutrophication.
JLES-Vol-14-No-P-93-97, December 2019.
AbstractA study was conducted on four shallow water ponds from January 2012 to December 2013 to determine the cyanobacterial bloom and its toxicity. Bloom samples were collected and filtered through a glass fiber filter. Filtered cells were extracted with Methanol-water and extracted sample was analyzed by High Performance Liquid Chromatography (HPLC) with UV, MS and MS-MS detections. Five types of microcystins viz., MC-RR (5.4µg/L), MC-YR (1.14 µg/L), MC-LR (2.0 µg/L), MC-WR (0.46 µg/L) and dm-MC-RR (0.36 µg/L) and two types microcystins viz., MC-RR (1.0 mg/l) and dm-MC-RR (0.04 µg/l) were detected from Pond 3 and Pond 4, respectively. MC-LR was found 2.0µg/g which is high than that of the provisional guideline. Very little amount of microcystins (1.04 µg/L) was found in Pond 4. Numerous MC congeners were found in an algal bloom but very little work has been done on the estimation of toxicological potency in bloom episode. More research is required to elucidate the lethal toxicity of different MC variants
Affan A, Khan S, Imokawa M and Ueno Y 2001. Determination of microcystins in natural waters of Bangladesh by ELISA, In: 6th Asian Fisheries forum book of abstracts, 124.
Affan A, Khomayis HS, Al-Harbi SA, Haque M and Khan S 2015. Effects of Environmental factors on Cyanobacterial Abundance and Cyanotoxins production in natural and drinking water. Pak. J. Biol. Sci. 18(2): 50-58.
Ahmed MS 2009. Isolation and characterization of microcystins (Heptapeptides Hepatotoxins) from Microcystisaeruginosa bloom in a homestead pond, Dhaka, Bangladesh. Res. J. Environ. Sci. 3: 245-250.
Ahmed MS, Hiller S and Luckas B 2007. Microcystisaeruginosa bloom and the occurrence of microcystins (Heptapeptieds Hepatotoxins) from an aquaculture pond in Gazipur, Bangladesh. Turkish J. Fisheri. Aquat. Sci. 8: 37–41.
Ahmed MS, Krueger T and Luckas B 2014. Anabaena sp. bloom and the occurrence of microcystin-LR from a eutrophic pond in Bangladesh. Int. J. Fisheri. Aquatic Stud. 2(3): 14-17.
Ahmed MS, Reichelt M and Luckas B 2000. Microcystisaeruginosa bloom and occurrence of microcystin in freshwater pond in Bangladesh. In 9th International Conference on Harmful Algal Blooms, 7-11 Feb 2000, Tasmania, Australia.
Campos A and Vasconcelos V 2010. Molecular mechanisms of microcystin toxicity in animal cells. Int. J. Mol. Sci.11: 268-287.
Carbis CR, Mitchell GF, Anderson JW and McCauley I 1996. The effects of microcystins on the serum biochemistry of crap, Cyprinuscarpio L., when the toxins are administered by gavage, immersion and intraperitoneal routes. J. Fish Dis. 19: 151-159.
Carmichael WW 1994. The toxins of cyanobacteria. Scienti.
American 270(1): 78-86.
Cazenave J, Wunderlin DA, Bistoni MA, Amé MV, Krause E, Pflugmacher S and Wiegand C 2005. Uptake, tissue distribution and accumulation of microcystin-RR in Corydoraspaleatus, Jenynsiamultidentata and Odontesthesbonariensis: a field and laboratory study. Aquat. Toxicol. 75: 178-190.
Chorus I 2005. Current Approaches to Cyanotoxin Risk Assessment, Risk Management and Regulations in Different Countries. Federal Environmental Agency (Umweltbundesamt), Berlin.
Codd GA, Morrison LF and Metcalf JS 2005. Cyanobacterial toxins: risk management for health protection. Toxicolo. Appli. Pharmacolo. 203(3): 264-272.
Dawson RM 1998. The toxicology of microcystins. Toxicon
16: 953-962.
Deblois CP, Aranda-Rodriguez R, Giani A and Bird DF 2008. Microcystin accumulation in liver and muscle of tilapia in two large Brazilian hydroelectric reservoirs. Toxicon 51: 435-448.
Eriksson JE, Toivola D, Meriluoto JA, Karaki H, Han YG and Hartshorne D 1990. Hepatocyte deformation induced by cyanobacterial toxins reflects inhibition of protein phosphatases. Biochem. Biophys. Res. Commun.173: 1347-1353.
Falconer I, Bartram J, Chorus I, Kuiper-Goodman T, Utkilen H, Burch M, Codd G 1999. Safe levels and safe practices. In: Toxic Cyanobacteria in Water: A Guide to Their Public Health Consequences, Monitoring and Management Chorus, Bartram,
- (Eds.). E & FN Spon, London.
Falconer IR 1991. Tumor promotion and liver injury caused by oral consumption of cyanobacteria. Environ. Toxicol. Water Quality 6: 177-184.
Fastner J, Codd GA, Metcalf JS, Woitke P, Wiedner C, and Utkilen H 2002. An international comparison exercise for the determination of purified microcystin-LR and microcystins in field material. Anal. Bioanal.Chem. 374: 437–444.
Fastner J, Heinze R and Chorus I 1995. Microcystin-content, hepatotoxicity and cytotoxicity of cyanobacteria in some German water bodies. Water Sci. Tech. 32: 165-170.
Fitzgerald DJ, Cunliffe DA and Burch MD 1999. Development of health alerts for cyanobacteria and related toxins in drinking water in South Australia. Environ. Toxicolo. 14(1): 203-209.
FRSS 2014. Fisheries Statistical Yearbook of Bangladesh. Fisheries Resources Survey System (FRSS), Department of Fisheries, Bangladesh. 30: pp. 52
Gupta N, Pant SC, Vijayaraghavan R and Lakshmana PV 2003. Comparative toxicity evaluation of cyanobacterial cyclic peptide toxin microcystin variants (LR, RR, YR) in mice. Toxicology 188: 285–296.
Humpage AR and Falconer IR 2003. Oral toxicity of the cyanobacterial toxin cylindrospermopsin in male Swiss albino mice: Determination of no observed adverse effect level for deriving a drinking water guideline value. Environ. Toxicol. 18(2): 94-103.
Islam AKM 1991. Two centuries of plant studies in Bangladesh and adjacent Regions. Phycology. Asiatic Soc. Bangladesh 97-153.
Jahan R, Khan S, Haque MM and Choi JK 2010. Study of harmful algal blooms in a eutrophic pond, Bangladesh. Environ. Monit. Assess. 170, 7-21.
Jewel MAS, Affan MA and Khan S 2003. Fish mortality due to cyanobacterial bloom in an aquaculture pond in Bangladesh. Pakistan J. Biol. Sci. 6(2): 1046–1050.
Lawton LA, Beattie KA, Hawser SP, Campbell DL and Codd GA 1994. Evaluation of assay methods for the determination of cyanobacterial hepatotoxicity. In: Codd GA, Jefferies TM, Keevil CW and Potter E.eds. Detection Methods for Cyanobacterial Toxins 1994. The Royal Society of Chemistry, Cambridge, pp. 111-116.
Lei H, Xie P, Chen J, Liang G, Yu T and Jiang Y 2008. Tissue distribution and depuration of the extracted hepatotoxic cyanotoxinmicrocystins in Crucian Crap (Carassiuscarassius) intraperitoneally injected at a sublethal dose. The Scient. World J. 8: 713-719.
Magalhães VF, Marinho MM, Domingos P, Oliveira AC, Costa SM, Azevedo LO and Azevedo SMFO 2003. Microcystins (cyanobacteria hepatotoxins) bioaccumulation in fish and crustaceans from Sepetiba Bay (Brasil, RJ). Toxicon. 42: 289–95
Mohammed ZA, Carmichael WW and Hussein AA 2003. Estimation of Microcystins in the freshwater fish Oreochromisniloticus in an Egyptian fish farm containing a Microcystis bloom. Environ. Toxicol. 18: 137–141.
Rastogi RP, Madamwar D and Incharoensakdi A 2015. Bloom dynamics of cyanobacteria and their toxins: Environmental Health Impacts and Mitigation Strategies. Front. Microbiol. 6: 1254.
Rastogi RP, Sinha RP and Incharoensakdi A 2014. The cyanotoxin- microcystins: current overview. Rev. Environ. Sci.Bio/Technol. 13: 215-149.
Runnegar MTC, Gerdes RG and Falconer IR 1991. The uptake of the cyanobacterial hepatotoxin microcystin by isolated rat hepatocytes. Toxicon 29: 43-51.
Svrcek C and Smith DW 2004. Cyanobacteria toxins and the current state of knowledge on water treatment options: a review. J. Environ. Engine. Sci. 3(3): 155-185.
Ueno Y, Nagata S, Tsutsumi T, Hasegawa A, Watanabe MF, Park HD, Chen G and Yu SZ 1996. Detection of microcystins, a bluegreen algae hepatotoxin, in drinking water sampled in Haimen and Fusui, endemic areas primary liver cancer in China, by highly sensitive immunoassay. Carcinogenesis 17: 1317- 1321.
Van Apeldoorn ME, Egmond HP, Speijers GJA and Bakker GJI 2007. Toxins of cyanobacteria. Molecul. Nutri. Food Res. 51(1): 7-60.
Welker M, Chorus I and Fastner J 2004.Occurrence of cyanobacterial toxins (microcystins) in surface waters of rural Bangladesh-pilot study. WHO Report, 23.
WHO 1998. Guidelines for drinking water quality, Health criteria and other supporting information. (2nd ed., Addendum Vol. 2) World Health Organization, Geneva, Switzerland.
Xie L, Xie P, Guo L, Li L, Miyabara Y and Park HD 2005. Organ distribution and bioaccumulation of MCs in freshwater fish at different trophic levels from the eutrophic Lake Chaohu, China. Environ. Toxicol. 20: 293-300.
Zhang D, Xie P and Chen J 2010. Effect of temperature on the stability of microcystins in muscle of fish and its consequences for food safety. Bull. Environ.Contam. Toxicol. 84: 202-207.
Zhou L, Yu H and Chen K 2002. Relationship between microcystin in drinking water and colorectal cancer. Biomed. Environ. Sci. 15(2):166-71
Sumaiya Ahmed, Sarah Subhana Islam , Md. Sagir Ahmed
DETERMINATION OF MICROCYSTINS FROM FRESHWATER PONDS IN MYMENSINGH, BANGLADESH
JLES-Vol-14-No-P-93-97, December 2019.
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