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OPTIMIZATION OF CULTURE CONDITIONS FOR BIOFILM FORMATION BY STAPHYLOCOCCUS AUREUS AND STENOTROPHOMONAS MALTOPHILIA
By: Farah Ulfat, Farzana Sayed Sraboni, Swagota Briti Ray Gupta, Md. Salah Uddin, Shahriar Zaman, Md. Abu Saleh
Key Words: Biofilm, pH, temperature, inoculum, antibiotics, ciprofloxacin.
JLES-Vol-18-No-P-1-8, December 2023.
AbstractThe microbes develop a special structure known as a biofilm to survive harsh environmental conditions, including well-known antibiotics. In this study, biofilm formation by two pathogenic bacterial strains, Staphylococcus aureus and Stenotrophomonas maltophilia was investigated under various cultural conditions, including growth medium, pH, temperature, and inoculum concentrations. The development of biofilms on the other media was generally higher than on glucose-based media. Biofilm development was most prominently observed at a neutral pH of 7.0, while acidic pH level did not promote biofilm growth. Comparatively, biofilm resilience increased as the temperature rose from 30 °C to 37 °C when compared to higher temperatures like 40 °C. A faster biomass plateau was achieved at higher bacterial concentrations as an effect of inoculum concentration. Out of the ten antibiotics examined in this research, Staphylococcus aureus appeared to be resistant to six of them, while Stenotrophomonas maltophilia was found to be resistant to four of them. This investigation also demonstrated that higher doses of the antibiotic administered over an extended period can potentially eradicate biofilms; a commonly found antibiotic, ciprofloxacin, was used for this analysis. A better understanding of these factors could enable more effective control of biofilm formation, either through prevention or elimination of biofilms or by implementing appropriate hazard analysis.
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Farah Ulfat, Farzana Sayed Sraboni, Swagota Briti Ray Gupta, Md. Salah Uddin, Shahriar Zaman, Md. Abu Saleh
OPTIMIZATION OF CULTURE CONDITIONS FOR BIOFILM FORMATION BY STAPHYLOCOCCUS AUREUS AND STENOTROPHOMONAS MALTOPHILIA
JLES-Vol-18-No-P-1-8, December 2023.
https://jlescience.com/les-publish/optimization-of-culture-conditions-for-biofilm-formation-by-staphylococcus-aureus-and-stenotrophomonas-maltophilia/Copyright © 2023
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